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p jnk  (Affinity Biosciences)

 
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    Structured Review

    Affinity Biosciences p jnk
    P Jnk, supplied by Affinity Biosciences, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/p+jnk/anti+jnk/pmc12914111-39-61-70
    Average 86 stars, based on 1 article reviews
    p jnk - by Bioz Stars, 2026-10
    86/100 stars

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    Related Articles

    Pyrolysis Gas Chromatography:

    Article Title: Network pharmacology reveals that Yanghe Decoction inhibits osteosarcoma progression via ROS-induced mitochondrial dysfunction and enhances cisplatin sensitivity
    Article Snippet: Cyclin B (#4138), Vimentin (#5741), N-cadherin (#13116), p38 (#9212), and p-p38 (#4511S) antibodies were purchased from Cell Signaling Technology (USA). .. Cytochrome c (CYT-C) (#AF0146), cleaved-PARP (#AF7023), Bcl-2 (#AF6139), cleaved-caspase 9 (#AF5240), cleaved-caspase 3 (#AF7022), matrix metalloproteinase 2 (MMP2) (#AF5330), MMP7 (#AF0218), peroxisome proliferator-activated receptor-γ-coactivator 1α (PGC-1α) (#AF5395), nuclear respiratory factor 1 (NRF1) (#AF5298), NRF2 (#AF0639), mitochondrial transcription factor A (TFAM) (#AF0531), PI3K (#AF6241), p-PI3K (#AF3242), AKT (#AF6261), p-AKT (#AF0016), extracellular signal-related kinase (ERK) (#AF0155), Jun N-terminal kinase (JNK) (#AF6318), p-ERK (#AF1015), p-JNK (#AF3318), and Snail (#AF6032) antibodies were purchased from Affinity Biosciences (USA). ..

    other:

    Article Title: Xiaoyao San against depression via glia-mediated neuroinflammation using network pharmacology and experimental validation
    Article Snippet: JNK (# AF6318) and p-JNK (Thr183 + Tyr185, # AF3318) were purchased from Affinity Biosciences (Jiangsu, China).

    Article Title: Integrating network pharmacology and experimental validation strategies to investigate the mechanisms and key flavonoids in medicinal and edible citrus plants against Alzheimer’s disease
    Article Snippet: JNK (# AF6318) and p-JNK (Thr183 + Tyr185, # AF3318) were purchased from Affinity Biosciences (Jiangsu, China).

    Incubation:

    Article Title: Targeting TNFAIP2 with NIR-II CRISPR-Cas9 nanosystem to overcome cisplatin resistance in laryngeal cancer
    Article Snippet: Equal amounts of protein were separated by SDS-PAGE and transferred to polyvinylidene fluoride (PVDF) membranes (FFP19, Beyotime, China). .. Membranes were blocked with 5% BSA (ST025-5g, Beyotime, China) for 1 h at room temperature and then incubated overnight at 4 °C with the following primary antibodies (dilution 1:1000 unless otherwise noted): anti-ABCG2 (rabbit, AF5177, Affbiotech, China), MDR1 (rabbit, AF5185, Affbiotech, China), CD44 (rabbit, DF6392, Affbiotech, China), CD133 (rabbit, AF5120, Affbiotech, China), Snail (rabbit, AF6032, Affbiotech, China), Slug (rabbit, AF4002, Affbiotech, China), Twist1 (rabbit, AF4009, Affbiotech, China), Vimentin (rabbit, AF7013, Affbiotech, China), E-cadherin (rabbit, AF0131, Affbiotech, China), ZO-1 (rabbit, AF5145, Affbiotech, China), JNK (rabbit, AF6318, Affbiotech, China), P-JNK (rabbit, AF3320, Affbiotech, China), Caspase 9 (rabbit, AF6348, Affbiotech, China), cl-Caspase 9 (rabbit, AF5244, Affbiotech, China), Caspase 3 (rabbit, AF6311, Affbiotech, China), cl-Caspase 3 (rabbit, AF7022, Affbiotech, China), NRF2 (rabbit, AF0639, Affbiotech, China), Cas9 (mouse, 0.5 μg/mL, A01935-40, Thermo Fisher, USA), and GAPDH (rabbit, AF7021, Affbiotech, China). .. After primary incubation, membranes were washed three times with PBST (0.1% Tween-20 in PBS, 5 min each) and incubated with secondary antibodies for 1 h at room temperature: goat anti-rabbit secondary antibody (Goat, 1:5000, S0001, Affbiotech, China) or goat anti-mouse secondary antibody (Goat, 1:5000, S0002, Affbiotech, China) at room temperature for 1 h. Following secondary incubation, membranes were washed again (3 × 5 min), then developed using ECL reagent (P0018S, Beyotime, China) and imaged by X-ray film exposure in a darkroom for 5–10 min.



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    Effect of Tax on gene expression of <t>the</t> <t>JNK</t> pathway in Cd-induced apoptosis of chicken primary kidney cells. (A) Molecular docking results between Tax and JNK. (B) Cellular Thermal Shift Assay to verify the interaction between Tax and JNK. (C) Relative protein expression of <t>p-JNK/JNK</t> and relative mRNA expression of JNK. * Indicates a significant difference between DMSO group and Cd group (n=10; * P < 0.05). # Indicates a significant difference between Cd + Tax group and Cd group ( n = 10; # P < 0.05). Results are presented as mean ± SEM. Statistical significance was determined by one-way ANOVA or unpaired Student’s t-test.
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    Image Search Results


    The information on the antibody.

    Journal: Aging Cell

    Article Title: Ghrelin Alleviates Aging‐Related Cognitive Impairment by Regulating Autophagy‐Related Signaling via the SHBG / JNK1 /Beclin1 Axis in Microglia

    doi: 10.1111/acel.70732

    Figure Lengend Snippet: The information on the antibody.

    Article Snippet: p‐JNK , 1:2000 , 80024‐1‐RR , Rabbit , Proteintech , USA.

    Techniques:

    Effect of Tax on gene expression of the JNK pathway in Cd-induced apoptosis of chicken primary kidney cells. (A) Molecular docking results between Tax and JNK. (B) Cellular Thermal Shift Assay to verify the interaction between Tax and JNK. (C) Relative protein expression of p-JNK/JNK and relative mRNA expression of JNK. * Indicates a significant difference between DMSO group and Cd group (n=10; * P < 0.05). # Indicates a significant difference between Cd + Tax group and Cd group ( n = 10; # P < 0.05). Results are presented as mean ± SEM. Statistical significance was determined by one-way ANOVA or unpaired Student’s t-test.

    Journal: Poultry Science

    Article Title: Cadmium-induced apoptosis via ROS/JNK pathway in chicken primary kidney cells and antagonism of taxifolin

    doi: 10.1016/j.psj.2026.106920

    Figure Lengend Snippet: Effect of Tax on gene expression of the JNK pathway in Cd-induced apoptosis of chicken primary kidney cells. (A) Molecular docking results between Tax and JNK. (B) Cellular Thermal Shift Assay to verify the interaction between Tax and JNK. (C) Relative protein expression of p-JNK/JNK and relative mRNA expression of JNK. * Indicates a significant difference between DMSO group and Cd group (n=10; * P < 0.05). # Indicates a significant difference between Cd + Tax group and Cd group ( n = 10; # P < 0.05). Results are presented as mean ± SEM. Statistical significance was determined by one-way ANOVA or unpaired Student’s t-test.

    Article Snippet: p-JNK , Wanleibio , 1:500 , WL01813.

    Techniques: Gene Expression, Thermal Shift Assay, Expressing

    The protein levels of cells treated with SP600125 and NAC. (A) Protein expression of p-JNK/JNK, Bad, Bid, Bcl-2, Bax, Bak, DIABLO, XIAP, Caspase-3, and Caspase-9. (B) Quantitative analysis of p-JNK/JNK, Bad, Bid, Bcl-2, Bax, Bak, DIABLO, XIAP, Caspase-3, and Caspase-9 expression. Data are presented as mean ± SD (n = 10). * Indicates a significant difference on Cd + SP600125 or Cd + NAC vs Cd group ( n = 10; * P < 0.05). # Indicates a significant difference between Cd + SP600125 + Tax group and Cd + Tax group ( n = 10; # P < 0.05).

    Journal: Poultry Science

    Article Title: Cadmium-induced apoptosis via ROS/JNK pathway in chicken primary kidney cells and antagonism of taxifolin

    doi: 10.1016/j.psj.2026.106920

    Figure Lengend Snippet: The protein levels of cells treated with SP600125 and NAC. (A) Protein expression of p-JNK/JNK, Bad, Bid, Bcl-2, Bax, Bak, DIABLO, XIAP, Caspase-3, and Caspase-9. (B) Quantitative analysis of p-JNK/JNK, Bad, Bid, Bcl-2, Bax, Bak, DIABLO, XIAP, Caspase-3, and Caspase-9 expression. Data are presented as mean ± SD (n = 10). * Indicates a significant difference on Cd + SP600125 or Cd + NAC vs Cd group ( n = 10; * P < 0.05). # Indicates a significant difference between Cd + SP600125 + Tax group and Cd + Tax group ( n = 10; # P < 0.05).

    Article Snippet: p-JNK , Wanleibio , 1:500 , WL01813.

    Techniques: Expressing